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Applied and Environmental Microbiology, May 2009, p. 3370-3372, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00039-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Transferable 20-Kilobase Multiple Drug Resistance-Conferring R Plasmid (pKL0018) from a Fish Pathogen (Lactococcus garvieae) Is Highly Homologous to a Conjugative Multiple Drug Resistance-Conferring Enterococcal Plasmid{triangledown}

Takeshi Maki, Mudjekeewis D. Santos, Hidehiro Kondo, Ikuo Hirono, and Takashi Aoki*

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan

Received 8 January 2009/ Accepted 9 February 2009

Lactococcus garvieae, the causative agent of lactococcosis, has evolved strains that are highly resistant to antibiotics. Here, the 20,034-bp sequence of L. garvieae conjugative plasmid pKL0018 was determined. It contained two ermB genes and one tetS gene and a backbone more than 96% identical to that of pRE25, an Enterococcus faecalis plasmid from dry sausage.

* Corresponding author. Mailing address: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan. Phone: 81-03-5463-0556. Fax: 81-03-5463-0690. E-mail: aoki{at}kaiyodai.ac.jp

{triangledown} Published ahead of print on 13 February 2009.

Applied and Environmental Microbiology, May 2009, p. 3370-3372, Vol. 75, No. 10
0099-2240/09/$08.00+0     doi:10.1128/AEM.00039-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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