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Applied and Environmental Microbiology, June 2009, p. 3586-3592, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02683-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus{triangledown}

Phat L. Tran,1,2 Adrienne A. Hammond,1,{dagger} Thomas Mosley,3 Janette Cortez,2 Tracy Gray,2 Jane A. Colmer-Hamood,1 Mayank Shashtri,4 Julian E. Spallholz,5 Abdul N. Hamood,1 and Ted W. Reid1,2,3*

Department of Microbiology & Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas,1 Department of Ophthalmology & Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, Texas,2 Selenium, Ltd., Austin, Texas,3 Eburon Organics International, Inc., Lubbock, Texas,4 Department of Nutrition, Texas Tech University, Lubbock, Texas5

Received 24 November 2008/ Accepted 27 March 2009

Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphate-buffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.

* Corresponding author. Mailing address: Department of Ophthalmology & Visual Sciences, Texas Tech University Health Sciences Center, 3601 4th Street, Stop 7217, Lubbock, TX 79430. Phone: (806) 743-2417, ext. 262. Fax: (806) 743-2471. E-mail: ted.reid{at}ttuhsc.edu

{triangledown} Published ahead of print on 3 April 2009.

{dagger} Present address: Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, TX.

Applied and Environmental Microbiology, June 2009, p. 3586-3592, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02683-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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