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Applied and Environmental Microbiology, June 2009, p. 3745-3754, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.01599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of a Unique Class C Acid Phosphatase from Clostridium perfringens{triangledown}

Thomas J. Reilly,1,2* Deborah L. Chance,3 Michael J. Calcutt,1 John J. Tanner,4,5 Richard L. Felts,4 Stephen C. Waller,6 Michael T. Henzl,5 Thomas P. Mawhinney,5 Irene K. Ganjam,2 and William H. Fales1,2

Department of Veterinary Pathobiology,1 Veterinary Medical Diagnostic Laboratory,2 Departments of Molecular Microbiology and Immunology,3 Chemistry,4 Biochemistry, University of Missouri, Columbia, Missouri 65211,5 Division of Infectious Diseases, University of Kansas Medical Center, Kansas City, Kansas 661606

Received 11 July 2008/ Accepted 29 March 2009

Clostridium perfringens is a gram-positive anaerobe and a pathogen of medical importance. The detection of acid phosphatase activity is a powerful diagnostic indicator of the presence of C. perfringens among anaerobic isolates; however, characterization of the enzyme has not previously been reported. Provided here are details of the characterization of a soluble recombinant form of this cell-associated enzyme. The denatured enzyme was ~31 kDa and a homodimer in solution. It catalyzed the hydrolysis of several substrates, including para-nitrophenyl phosphate, 4-methylumbelliferyl phosphate, and 3' and 5' nucleoside monophosphates at pH 6. Calculated Kms ranged from 0.2 to 0.6 mM with maximum velocity ranging from 0.8 to 1.6 µmol of Pi/s/mg. Activity was enhanced in the presence of some divalent cations but diminished in the presence of others. Wild-type enzyme was detected in all clinical C. perfringens isolates tested and found to be cell associated. The described enzyme belongs to nonspecific acid phosphatase class C but is devoid of lipid modification commonly attributed to this class.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, Veterinary Medical Diagnostic Laboratory, University of Missouri, College of Veterinary Medicine, 1600 E. Rollins St., Columbia, MO 65211. Phone: (573) 884-9282. Fax: (573) 884-1981. E-mail: reillyt{at}missouri.edu

{triangledown} Published ahead of print on 10 April 2009.

Applied and Environmental Microbiology, June 2009, p. 3745-3754, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.01599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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