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Applied and Environmental Microbiology, June 2009, p. 3755-3764, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02964-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Composition of Bacterial Communities Associated with Natural and Laboratory Populations of Asobara tabida Infected with Wolbachia{triangledown} ,{dagger}

Karima Zouache, Denis Voronin, Van Tran-Van, and Patrick Mavingui*

Université de Lyon, F-69622 Lyon, Université Lyon 1, Villeurbanne, and CNRS, UMR5557, Ecologie Microbienne, Villeurbanne, France

Received 30 December 2008/ Accepted 3 April 2009

Asobara tabida wasps are fly endoparasitoids that naturally harbor three Wolbachia strains, which induce cytoplasmic incompatibility and control oogenesis. To investigate whether other bacteria play a role in wasp biology, we surveyed the bacterial communities of wild A. tabida populations originating from different regions of France and of laboratory colonies using PCR-denaturing gradient gel electrophoresis and culture methods. Proteobacteria and Firmicutes were found to be the main phyla represented in these populations. Among these were several cultured and uncultured representatives of the genera Acetobacter, Acidomonas, Bacillus, Brevibacillus, Duganella, Herbaspirillum, Pseudomonas, Staphylococcus, and Streptococcus. In addition to Wolbachia, wild individuals harbored Rickettsia, which tended to be lost when insects were reared in the laboratory. The antibiotic treatment used to generate wasp sublines singly infected with Wolbachia also affected the overall bacterial composition, with most fingerprint sequences being characteristic of the family Enterobacteriaceae. We also screened for potentially heritable endosymbionts by PCR and fluorescence in situ hybridization in stable laboratory lines, with only Wolbachia being consistently found in wasp ovaries.

* Corresponding author. Mailing address: UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne cedex, France. Phone: 33 4 72 43 11 43. Fax: 33 4 72 43 12 23. E-mail: patrick.mavingui{at}univ-lyon1.fr

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2009, p. 3755-3764, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02964-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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