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Applied and Environmental Microbiology, June 2009, p. 3800-3802, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.00341-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Translocation of a Thioether-Bridged Azurin Peptide Fragment via the Sec Pathway in Lactococcus lactis

Anneke Kuipers, Rick Rink, and Gert N. Moll^*

BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, The Netherlands

Received 11 February 2009/ Accepted 21 March 2009

This study demonstrates for the first time that a thioether-containing peptide, an azurin fragment, can be translocated via the Sec pathway. This methyl-lanthionine was introduced by the nisin modification enzymes. The Sec pathway can therefore be a successful alternative for those cyclized peptides that are inefficiently transported via NisT.

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* Corresponding author. Mailing address: BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Phone: 31 50 3638070. Fax: 31 50 3634429. E-mail: Moll{at}biomade.nl

Published ahead of print on 27 March 2009.

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Applied and Environmental Microbiology, June 2009, p. 3800-3802, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.00341-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.