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Applied and Environmental Microbiology, June 2009, p. 3813-3817, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.00048-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of GerKB in Germination and Outgrowth of Clostridium perfringens Spores{triangledown} ,{dagger}

Daniel Paredes-Sabja,1 Peter Setlow,3 and Mahfuzur R. Sarker1,2*

Department of Biomedical Sciences,1 Department of Microbiology, Oregon State University, Corvallis, Oregon 97331,2 Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 060303

Received 9 January 2009/ Accepted 29 March 2009

Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.

* Corresponding author. Mailing address: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 216 Dryden Hall, Corvallis, OR 97331. Phone: (541) 737-6918. Fax: (541) 737-2730. E-mail: sarkerm{at}oregonstate.edu

{triangledown} Published ahead of print on 10 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2009, p. 3813-3817, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.00048-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Paredes-Sabja, D., Udompijitkul, P., Sarker, M. R. (2009). Inorganic Phosphate and Sodium Ions Are Cogerminants for Spores of Clostridium perfringens Type A Food Poisoning-Related Isolates. Appl. Environ. Microbiol. 75: 6299-6305 [Abstract] [Full Text]  
  • Paredes-Sabja, D., Setlow, P., Sarker, M. R. (2009). GerO, a Putative Na+/H+-K+ Antiporter, Is Essential for Normal Germination of Spores of the Pathogenic Bacterium Clostridium perfringens. J. Bacteriol. 191: 3822-3831 [Abstract] [Full Text]  


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