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Applied and Environmental Microbiology, June 2009, p. 3818-3822, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02636-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Efficient Bioethanol Production by a Recombinant Flocculent Saccharomyces cerevisiae Strain with a Genome-Integrated NADP+-Dependent Xylitol Dehydrogenase Gene{triangledown}

Akinori Matsushika,1* Hiroyuki Inoue,1 Seiya Watanabe,2 Tsutomu Kodaki,2 Keisuke Makino,2 and Shigeki Sawayama1

Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan,1 Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan2

Received 18 November 2008/ Accepted 21 March 2009

The recombinant industrial Saccharomyces cerevisiae strain MA-R5 was engineered to express NADP+-dependent xylitol dehydrogenase using the flocculent yeast strain IR-2, which has high xylulose-fermenting ability, and both xylose consumption and ethanol production remarkably increased. Furthermore, the MA-R5 strain produced the highest ethanol yield (0.48 g/g) from nonsulfuric acid hydrolysate of wood chips.

* Corresponding author. Mailing address: Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. Phone and fax: 81-823-72-1936. E-mail: a-matsushika{at}aist.go.jp

{triangledown} Published ahead of print on 27 March 2009.

Applied and Environmental Microbiology, June 2009, p. 3818-3822, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02636-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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