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Applied and Environmental Microbiology, June 2009, p. 3823-3825, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02394-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

First Gene Cassettes of Integrons as Targets in Finding Adaptive Genes in Metagenomes{triangledown}

Lionel Huang, Christine Cagnon,* Pierre Caumette, and Robert Duran

Equipe Environnement et Microbiologie, IPREM UMR CNRS 5254, Université de Pau et des Pays de l'Adour, IBEAS, BP 1155, 64013 Pau Cedex, France

Received 17 October 2008/ Accepted 3 April 2009

The first gene cassettes of integrons are involved in the last adaptation response to changing conditions and are also the most expressed. We propose a rapid method for the selection of clones carrying an integron first gene cassette that is useful for finding adaptive genes in environmental metagenomic libraries.

* Corresponding author. Mailing address: Université de Pau et des Pays de l'Adour, IPREM UMR CNRS 5254, IBEAS, BP 1155, 64013 Pau Cedex, France. Phone: 33 5 59407480. Fax: 33 5 59407494. E-mail: christine.cagnon{at}univ-pau.fr

{triangledown} Published ahead of print on 10 April 2009.

Applied and Environmental Microbiology, June 2009, p. 3823-3825, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02394-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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