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 Previous Article

Applied and Environmental Microbiology, June 2009, p. 3826-3833, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02542-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation{triangledown}

Yoshiteru Aoi,1* Tomoyuki Kinoshita,2 Toru Hata,2 Hiroaki Ohta,3 Haruko Obokata,3 and Satoshi Tsuneda3

Waseda Institute for Advanced Study, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan,1 Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan,2 Department of Life Science and Medical Bio-Science, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan3

Received 7 November 2008/ Accepted 23 March 2009

A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

* Corresponding author. Mailing address: Waseda Institute for Advanced Study, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan. Phone: 81-3-5369-7325. Fax: 81-3-3341-2684. E-mail: yoshiteruaoi{at}aoni.waseda.jp

{triangledown} Published ahead of print on 27 March 2009.

Applied and Environmental Microbiology, June 2009, p. 3826-3833, Vol. 75, No. 11
0099-2240/09/$08.00+0     doi:10.1128/AEM.02542-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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