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Applied and Environmental Microbiology, June 2009, p. 3930-3936, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02487-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Effect of Amino Acid Availability on Vitamin B12 Production in Lactobacillus reuteri{triangledown} ,{dagger}

Filipe Santos,1,{ddagger} Bas Teusink,1,{ddagger} Douwe Molenaar,1,{ddagger} Maurice van Heck,1 Michiel Wels,1 Sander Sieuwerts,1 Willem M. de Vos,2 and Jeroen Hugenholtz1*

Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands,1 Laboratory of Microbiology, Wageningen University and Research Centre, Dreijenplein 10, 6703 HB Wageningen, The Netherlands2

Received 30 October 2008/ Accepted 9 April 2009

Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B12 from nearly undetectable levels (with omission of isoleucine) to levels 20-fold higher than the levels previously reported (with omission of cysteine). Using cDNA microarray experiments, we analyzed the transcriptional response of L. reuteri to medium lacking cysteine. The results supported the observed high level of B12 production and provided new avenues for future improvement of production of vitamin B12.

* Corresponding author. Mailing address: P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31 318 65 95 40. Fax: 31 318 65 04 00. E-mail: jeroen.hugenholtz{at}nizo.nl

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.

Applied and Environmental Microbiology, June 2009, p. 3930-3936, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02487-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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