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Applied and Environmental Microbiology, June 2009, p. 3937-3943, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.00432-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Susceptibility of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) to the Entomopathogenic Fungus Metarhizium anisopliae when Feeding on Bacillus thuringiensis Cry3Bb1-Expressing Maize{triangledown}

Michael Meissle, Christina Pilz,{dagger} and Jörg Romeis*

Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, CH-8046 Zurich, Switzerland

Received 20 February 2009/ Accepted 6 April 2009

Genetically engineered maize producing the insecticidal protein Cry3Bb1 from Bacillus thuringiensis (Bt maize) is protected against corn rootworms (Diabrotica spp.), which are serious maize pests in North America and Europe. The aim of the present study was to investigate the interaction of Bt maize (event MON88017) and the entomopathogenic fungus Metarhizium anisopliae for controlling the western corn rootworm, Diabrotica virgifera virgifera. Exposure to Cry3Bb1 expressed in Bt maize seedlings resulted in decreased weight gain in D. v. virgifera larvae but did not influence susceptibility to M. anisopliae. Adult beetles were not affected by Cry3Bb1 in their food, but mortality when feeding on maize leaves was higher than when feeding on silk. Adults were more susceptible to the fungus than larvae. The results indicate that the effects of Bt maize and M. anisopliae on D. v. virgifera are additive and that Bt maize does not interfere with the biological control provided by entomopathogenic fungi.

* Corresponding author. Mailing address: Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstrasse 191, CH-8046 Zurich, Switzerland. Phone: 41 44 377 72 99. Fax: 41 44 377 72 01. E-mail: joerg.romeis{at}art.admin.ch

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Present address: Austrian Agency for Health and Food Safety (AGES), Spargelfeldstrasse 191, 1220 Vienna, Austria.

Applied and Environmental Microbiology, June 2009, p. 3937-3943, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.00432-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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