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Applied and Environmental Microbiology, June 2009, p. 4197-4201, Vol. 75, No. 12
0099-2240/09/$08.00+0 doi:10.1128/AEM.00156-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain,1 Institut Pasteur, Unité de Génétique Moléculaire, and CNRS URA2172, Paris, France2
Received 22 January 2009/ Accepted 11 April 2009
The gene (cpo) encoding the extracellular protease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica CP76 was cloned, and its nucleotide sequence was analyzed. The cpo gene encodes a 733-residue protein showing sequence similarity to metalloproteases of the M4 family. The type II secretion apparatus was shown to be responsible for secretion of the haloprotease CPI.
Published ahead of print on 17 April 2009.
Supplemental material for this article may be found at http://aem.asm.org/.
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