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Applied and Environmental Microbiology, June 2009, p. 4202-4205, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02606-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces virginiae IBL-14{triangledown} ,{dagger}

Wei Wang, Feng-Qing Wang, and Dong-Zhi Wei*

State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China

Received 14 November 2008/ Accepted 10 April 2009

A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

* Corresponding author. Mailing address: State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Phone: 86-21-64252981. Fax: 86-21-64250068. E-mail: dzhwei{at}ecust.edu.cn

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, June 2009, p. 4202-4205, Vol. 75, No. 12
0099-2240/09/$08.00+0     doi:10.1128/AEM.02606-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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