This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Habib, I.
Right arrow Articles by De Zutter, L.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Habib, I.
Right arrow Articles by De Zutter, L.
Agricola
Right arrow Articles by Habib, I.
Right arrow Articles by De Zutter, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2009, p. 4277-4288, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02269-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Correlation between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping{triangledown}

Ihab Habib,1,6* Rogier Louwen,2 Mieke Uyttendaele,3 Kurt Houf,1 Olivier Vandenberg,4 Edward E. Nieuwenhuis,2 William G. Miller,5 Alex van Belkum,2 and Lieven De Zutter1

Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke,1 Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent,3 National Reference Centre for Enteric Campylobacters, Department of Microbiology, Saint-Pierre University Hospital, Rue Haute 322, B-1000 Brussels, Belgium,4 Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre,'s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands,2 Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture (USDA), ARS, WRRC, 800 Buchanan Street, Albany, California 94710,5 Food Hygiene and Control Division, High Institute of Public Health (HIPH), Alexandria University, 165 El-Horrya Avenue, Alexandria, Egypt6

Received 2 October 2008/ Accepted 26 April 2009

Significant interest in studying the lipooligosaccharide (LOS) of Campylobacter jejuni has stemmed from its potential role in postinfection paralytic disorders. In this study we present the results of PCR screening of five LOS locus classes (A, B, C, D, and E) for a collection of 116 C. jejuni isolates from chicken meat (n = 76) and sporadic human cases of diarrhea (n = 40). We correlated LOS classes with clonal complexes (CC) assigned by multilocus sequence typing (MLST). Finally, we evaluated the invasion potential of a panel of 52 of these C. jejuni isolates for Caco-2 cells. PCR screening showed that 87.1% (101/116) of isolates could be assigned to LOS class A, B, C, D, or E. Concordance between LOS classes and certain MLST CC was revealed. The majority (85.7% [24/28]) of C. jejuni isolates grouped in CC-21 were shown to express LOS locus class C. The invasion potential of C. jejuni isolates possessing sialylated LOS (n = 29; classes A, B, and C) for Caco-2 cells was significantly higher (P < 0.0001) than that of C. jejuni isolates with nonsialylated LOS (n = 23; classes D and E). There was no significant difference in invasiveness between chicken meat and human isolates. However, C. jejuni isolates assigned to CC-206 (correlated with LOS class B) or CC-21 (correlated with LOS class C) showed statistically significantly higher levels of invasion than isolates from other CC. Correlation between LOS classes and CC was further confirmed by pulsed-field gel electrophoresis. The present study reveals a correlation between genotypic diversity and LOS locus classes of C. jejuni. We showed that simple PCR screening for C. jejuni LOS classes could reliably predict certain MLST CC and add to the interpretation of molecular-typing results. Our study corroborates that sialylation of LOS is advantageous for C. jejuni fitness and virulence in different hosts. The modulation of cell surface carbohydrate structure could enhance the ability of C. jejuni to adapt to or survive in a host.

* Corresponding author. Mailing address: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 09 264 73 41. Fax: 32 09 264 74 91. E-mail: ihab.habib{at}ugent.be

{triangledown} Published ahead of print on 1 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4277-4288, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02269-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Habib, I., Miller, W. G., Uyttendaele, M., Houf, K., De Zutter, L. (2009). Clonal Population Structure and Antimicrobial Resistance of Campylobacter jejuni in Chicken Meat from Belgium. Appl. Environ. Microbiol. 75: 4264-4272 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»