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Applied and Environmental Microbiology, July 2009, p. 4616-4619, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00519-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Versatile Solidified Nanofibrous Cellulose-Containing Media for Growth of Extremophiles{triangledown}

Mikiko Tsudome,1 Shigeru Deguchi,1* Kaoru Tsujii,2 Susumu Ito,3 and Koki Horikoshi1

Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan,1 Nanotechnology Research Center, Research Institute for Electronic Science, Hokkaido University, N21, W10, Kita-ku, Sapporo 001-0021, Japan,2 Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihra-cho, Okinawa 903-0213, Japan3

Received 2 March 2009/ Accepted 27 April 2009

Solidified media that employ a porous matrix of nanofibrous cellulose are described. The physicochemical stability of the porous structure allows the development of solidified media that can support the growth of extremophiles, such as acidophilic Acidiphilium, alkaliphilic Bacillus, thermophilic Geobacillus and Thermus, alkalithermophilic Bacillus, and acidothermophilic Sulfolobus microbes. The cellulose-supported media have several advantages over agar- and gellan gum-derived media, including versatility and stability.

* Corresponding author. Mailing address: Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Phone: 81-46-867-9679. Fax: 81-46-867-9715. E-mail: shigeru.deguchi{at}jamstec.go.jp

{triangledown} Published ahead of print on 1 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4616-4619, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00519-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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