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Applied and Environmental Microbiology, July 2009, p. 4624-4626, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02916-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Retention and Release of Cryptosporidium parvum Oocysts by Experimental Biofilms Composed of a Natural Stream Microbial Community{triangledown}

E. A. Wolyniak,1* B. R. Hargreaves,1 and K. L. Jellison2

Department of Earth and Environmental Science, Lehigh University, Bethlehem, Pennsylvania,1 Department of Civil and Environmental Engineering, Lehigh University, Bethlehem, Pennsylvania2

Received 22 December 2008/ Accepted 6 May 2009

Cryptosporidium parvum oocysts accumulate on biofilm surfaces. The percentage of oocysts attached to biofilms remained nearly constant while oocysts were supplied to the system but decreased to a new steady-state level once oocysts were removed from the feed. More oocysts attached to summer biofilm cultures than winter biofilm cultures.


* Corresponding author. Mailing address: Department of Earth and Environmental Science, Lehigh University, 31 Williams Drive, Bethlehem, PA 18015. Phone: (610) 758-4901. Fax: (610) 758-3677. E-mail: eaw205{at}lehigh.edu

{triangledown} Published ahead of print on 15 May 2009.


Applied and Environmental Microbiology, July 2009, p. 4624-4626, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.02916-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.