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Applied and Environmental Microbiology, July 2009, p. 4636-4640, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00548-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

High-Level Biosynthesis of the Anteiso-C17 Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity{triangledown}

Patrick Fickers,1,{dagger}* Jean-Sébastien Guez,2,{dagger} Christian Damblon,3 Valérie Leclère,2 Max Béchet,2 Philippe Jacques,2 and Bernard Joris1

Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, Bat B6, B-4000 Liège, Belgium,1 Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM, UPRES EA 1026), Polytech'Lille, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq Cedex, France,2 Chimie Biologique Structurale, Université de Liège, Institut de Chimie, Bat B6, B-4000 Liège, Belgium3

Received 6 March 2009/ Accepted 29 April 2009

High-level production (880 mg liter–1) and isolation of the anteiso-C17 isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.

* Corresponding author. Mailing address: Centre d'Ingénierie des Protéines, Laboratoire de Physiologie et Génétique Bactérienne, Université de Liège, Institut de Chimie, Bat B6, B-4000 Liège, Belgium. Phone: 32 4 366 3348. Fax: 32 4 366 3364. E-mail: pfickers{at}ulg.ac.be

{triangledown} Published ahead of print on 8 May 2009.

{dagger} Both authors contributed equally to this article.

Applied and Environmental Microbiology, July 2009, p. 4636-4640, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00548-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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