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Applied and Environmental Microbiology, July 2009, p. 4644-4647, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00283-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of a Novel Toluene-Degrading Bacterium from the Candidate Phylum TM7, as Determined by DNA Stable Isotope Probing{triangledown}

Chunling Luo,1 Shuguang Xie,2 Weimin Sun,3 Xiangdong Li,1 and Alison M. Cupples3*

Department of Civil and Structural Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China,1 College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China,2 Department of Civil and Environmental Engineering, Michigan State University, East Lansing, Michigan 488243

Received 4 February 2009/ Accepted 7 May 2009

The dominant bacterium responsible for carbon uptake from toluene in an agricultural soil was identified by stable isotope probing. Samples were amended with unlabeled toluene or labeled [ring-13C6]toluene, and DNA was extracted over time. Sequencing indicated that the organism involved belongs to the candidate phylum TM7. Microorganisms in this candidate phylum are of particular interest because although they have been found in a variety of habitats, no stable culture of any species exists, so their general metabolic capabilities are largely unknown.

* Corresponding author. Mailing address: A129 Research Engineering Complex, Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824. Phone: (517) 432-3370. Fax: (517) 355-0250. E-mail: cupplesa{at}msu.edu

{triangledown} Published ahead of print on 15 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4644-4647, Vol. 75, No. 13
0099-2240/09/$08.00+0     doi:10.1128/AEM.00283-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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