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Applied and Environmental Microbiology, July 2009, p. 4897-4900, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00686-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Effects of Bacillus thuringiensis {delta}-Endotoxins on the Pea Aphid (Acyrthosiphon pisum){triangledown}

Manuel Porcar,1* Anne-Marie Grenier,2 Brian Federici,3 and Yvan Rahbé2

Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Apartado Postal 22085, 46071 València, Spain,1 Université de Lyon, INRA, IFR41 Bioenvironnement et Santé, UMR203 INRA INSA-Lyon BF2I, Biologie Fonctionnelle Insectes et Interactions, Bat. L.-Pasteur, 20 Ave. A. Einstein, F-69621 Villeurbanne Cedex, France,2 Department of Entomology and Interdepartmental Graduate Programs in Genetics and Microbiology, University of California at Riverside, Riverside, California 925213

Received 24 March 2009/ Accepted 11 May 2009

Four Bacillus thuringiensis {delta}-endotoxins, Cry3A, Cry4Aa, Cry11Aa, and Cyt1Aa, were found to exhibit low to moderate toxicity on the pea aphid, Acyrthosiphon pisum, in terms both of mortality and growth rate. Cry1Ab was essentially nontoxic except at high rates. To demonstrate these effects, we had to use exhaustive buffer-based controls.


* Corresponding author. Mailing address: Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, 46071 Valencia, Spain. Phone: (34) 963 544 473. Fax: (34) 963 543 670. E-mail: manuel.porcar{at}uv.es

{triangledown} Published ahead of print on 15 May 2009.


Applied and Environmental Microbiology, July 2009, p. 4897-4900, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00686-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.