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Applied and Environmental Microbiology, July 2009, p. 4901-4903, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00344-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Construction and Characterization of Three Lactate Dehydrogenase-Negative Enterococcus faecalis V583 Mutants{triangledown}

Maria Jönsson,* Zhian Saleihan, Ingolf F. Nes, and Helge Holo

Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway

Received 11 February 2009/ Accepted 13 May 2009

The roles of the two ldh genes of Enterococcus faecalis were studied using knockout mutants. Deletion of ldh-1 causes a metabolic shift from homolactic fermentation to ethanol, formate, and acetoin production, with a high level of formate production even under aerobic conditions. Ldh-2 plays only a minor role in lactate production.

* Corresponding author. Mailing address: Department of Chemistry, Biotechnology and Food Science, The Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway. Phone: 47 6496 6248. Fax: 47 6496 5901. E-mail: maria.jonsson{at}umb.no

{triangledown} Published ahead of print on 22 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4901-4903, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00344-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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