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Applied and Environmental Microbiology, July 2009, p. 4913-4918, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00246-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Quantitative Real-Time PCR Assay for QPX (Thraustochytriidae), a Parasite of the Hard Clam (Mercenaria mercenaria){triangledown}

Qianqian Liu, Bassem Allam, and Jackie L. Collier*

School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, New York 11794-5000

Received 1 February 2009/ Accepted 15 May 2009

We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

* Corresponding author. Mailing address: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY 11794-5000. Phone: (631) 632-8696. Fax: (631) 632-8915. E-mail: jcollier{at}notes.cc.sunysb.edu

{triangledown} Published ahead of print on 22 May 2009.

Applied and Environmental Microbiology, July 2009, p. 4913-4918, Vol. 75, No. 14
0099-2240/09/$08.00+0     doi:10.1128/AEM.00246-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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