This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Marti, R.
Right arrow Articles by Pourcher, A.-M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marti, R.
Right arrow Articles by Pourcher, A.-M.
Agricola
Right arrow Articles by Marti, R.
Right arrow Articles by Pourcher, A.-M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2009, p. 4967-4974, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.02791-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups{triangledown}

Romain Marti,1 Patrick Dabert,1 and Anne-Marie Pourcher1,2*

Cemagref, UR GERE, 17 Avenue de Cucillé, 35044 Rennes Cedex, France,1 Laboratoire d'Etudes Environnementales des Systèmes Anthropisés, 2 Boulevard Lavoisier, 49045 Angers Cedex 01, France2

Received 8 December 2008/ Accepted 5 June 2009

The objective of this study was to identify a microbial marker for pig manure contamination. We quantified the persistence of four dominant bacterial groups from the pig intestinal tract throughout manure handling at 10 livestock operations (including aerobic digestion) by using molecular typing. The partial 16S rRNA genes of Bacteroides-Prevotella, Eubacterium-Clostridiaceae, Bacillus-Streptococcus-Lactobacillus (BSL), and Bifidobacterium group isolates were amplified and analyzed by capillary electrophoresis single-strand conformation polymorphism. The most dominant bacterial populations were identified by cloning and sequencing their 16S rRNA genes. The results showed that Bifidobacterium spp. and, to a lesser extent, members of the BSL group, were less affected by the aerobic treatment than either Eubacterium-Clostridiaceae or Bacteroides-Prevotella. Two Bifidobacterium species found in raw manure were still present in manure during land application, suggesting that they can survive outside the pig intestinal tract and also survive aerobic treatment. The 16S-23S rRNA internal transcribed spacer of one species, Bifidobacterium thermacidophilum subsp. porcinum, was sequenced, and a specific pair of primers was designed for its detection in the environment. With this nested PCR assay, this potential marker was not detected in samples from 30 bovine, 30 poultry, and 28 human fecal samples or in 15 urban wastewater effluents. As it was detected in runoff waters after spreading of pig manure, we propose this marker as a suitable microbial indicator of pig manure contamination.

* Corresponding author. Mailing address: Cemagref, UR GERE, 17 Avenue de Cucillé, 35044 Rennes Cedex, France. Phone: 33 2 23 48 21 37. Fax: 33 2 23 48 21 15. E-mail: anne-marie.pourcher{at}cemagref.fr

{triangledown} Published ahead of print on 12 June 2009.

Applied and Environmental Microbiology, August 2009, p. 4967-4974, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.02791-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»