This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Fournier, D.
Right arrow Articles by Hatzinger, P. B.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fournier, D.
Right arrow Articles by Hatzinger, P. B.
Agricola
Right arrow Articles by Fournier, D.
Right arrow Articles by Hatzinger, P. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2009, p. 5088-5093, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00418-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Aerobic Biodegradation of N-Nitrosodimethylamine by the Propanotroph Rhodococcus ruber ENV425{triangledown}

Diane Fournier,1 Jalal Hawari,1 Annamaria Halasz,1 Sheryl H. Streger,2 Kevin R. McClay,2 Hisako Masuda,3 and Paul B. Hatzinger2*

Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada,1 Shaw Environmental, Inc., Lawrenceville, New Jersey 08648,2 Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, New Jersey 089013

Received 19 February 2009/ Accepted 5 June 2009

The propanotroph Rhodococcus ruber ENV425 was observed to rapidly biodegrade N-nitrosodimethylamine (NDMA) after growth on propane, tryptic soy broth, or glucose. The key degradation intermediates were methylamine, nitric oxide, nitrite, nitrate, and formate. Small quantities of formaldehyde and dimethylamine were also detected. A denitrosation reaction, initiated by hydrogen atom abstraction from one of the two methyl groups, is hypothesized to result in the formation of n-methylformaldimine and nitric oxide, the former of which decomposes in water to methylamine and formaldehyde and the latter of which is then oxidized further to nitrite and then nitrate. Although the strain mineralized more than 60% of the carbon in [14C]NDMA to 14CO2, growth of strain ENV425 on NDMA as a sole carbon and energy source could not be confirmed. The bacterium was capable of utilizing NDMA, as well as the degradation intermediates methylamine and nitrate, as sources of nitrogen during growth on propane. In addition, ENV425 reduced environmentally relevant microgram/liter concentrations of NDMA to <2 ng/liter in batch cultures, suggesting that the bacterium may have applications for groundwater remediation.

* Corresponding author. Mailing address: Shaw Environmental, Inc., 17 Princess Road, Lawrenceville, NJ 08648. Phone: (609) 895-5356. Fax: (609) 895-1858. E-mail: paul.hatzinger{at}shawgrp.com

{triangledown} Published ahead of print on 19 June 2009.

Applied and Environmental Microbiology, August 2009, p. 5088-5093, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00418-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»