This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Jesenská, A.
Right arrow Articles by Damborsky, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jesenská, A.
Right arrow Articles by Damborsky, J.
Agricola
Right arrow Articles by Jesenská, A.
Right arrow Articles by Damborsky, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2009, p. 5157-5160, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00199-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily{triangledown}

Andrea Jesenská,1 Marta Monincová,1 Tána Koudeláková,1 Khomaini Hasan,1 Radka Chaloupková,1 Zbynek Prokop,1 Arie Geerlof,2 and Jirí Damborsky1*

Loschmidt Laboratories, Institute of Experimental Biology and National Center for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5/A4, 625 00 Brno, Czech Republic,1 EMBL—Hamburg Outstation, DESY, Building 25A, Notkestraße 85, 22603 Hamburg, Germany2

Received 28 January 2009/ Accepted 30 May 2009

This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenase genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structures and very low activities with typical substrates of haloalkane dehalogenases.

* Corresponding author. Mailing address: Loschmidt Laboratories, Institute of Experimental Biology and National Center for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5/A4, 625 00 Brno, Czech Republic. Phone: 420-5-4949 3467. Fax: 420-5-4949 2556. E-mail: jiri{at}chemi.muni.cz

{triangledown} Published ahead of print on 5 June 2009.

Applied and Environmental Microbiology, August 2009, p. 5157-5160, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00199-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»