This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Chen, I-C.
Right arrow Articles by Hsu, W.-H.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, I-C.
Right arrow Articles by Hsu, W.-H.
Agricola
Right arrow Articles by Chen, I-C.
Right arrow Articles by Hsu, W.-H.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2009, p. 5161-5166, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00074-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Novel Lysine Racemase from a Soil Metagenomic Library{triangledown} ,{dagger}

I-Chien Chen,1 Wei-De Lin,1 Shin-Kuang Hsu,2 Venkatesan Thiruvengadam,1 and Wen-Hwei Hsu3*

Institute of Molecular Biology, College of Life Sciences, National Chung Hsing University, Taichung, Taiwan 402,1 Department of Medical Research, China Medical University Hospital, Taichung, Taiwan 404,2 Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan 4063

Received 13 January 2009/ Accepted 1 June 2009

A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and D-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the L-lysine-to-D-lysine direction than in the reverse reaction.

* Corresponding author. Mailing address: Institute of Molecular Biology, College of Life Sciences, National Chung Hsing University, 250 Kuo-Kuang Rd., Taichung, Taiwan 402. Phone: 886-4-2285-1885. Fax: 886-4-2287-4879. E-mail: whhsu{at}dragon.nchu.edu.tw

{triangledown} Published ahead of print on 5 June 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2009, p. 5161-5166, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00074-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»