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Applied and Environmental Microbiology, August 2009, p. 5167-5169, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00036-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of an mlrA Gene-Directed TaqMan PCR Assay for Quantitative Assessment of Microcystin-Degrading Bacteria within Water Treatment Plant Sand Filter Biofilms{triangledown}

Daniel Hoefel,1,2* Caroline M. M. Adriansen,3 Magali A. C. Bouyssou,3 Christopher P. Saint,1,2 Gayle Newcombe,1 and Lionel Ho1,2

Australian Water Quality Centre, South Australian Water Corporation, 250 Victoria Square, South Australia 5000, Australia,1 School of Earth and Environmental Sciences, University of Adelaide, South Australia 5005, Australia,2 École Nationale Supérieure D'Ingénieurs De Limoges, 16 Rue Atlantis—Parc Ester, B.P. 6804, F87068 Limoges Cedex, France3

Received 7 January 2009/ Accepted 1 June 2009

We report for the first time a quantitative mlrA gene-directed TaqMan PCR assay for the rapid detection of microcystin-degrading bacteria. This was applied, in combination with 16S ribosomal DNA-directed quantitative PCR and denaturing gradient gel electrophoresis, to study virgin sand filter column biofilm development and to correlate mlrA gene abundance with microcystin removal efficiency.

* Corresponding author. Mailing address: Australian Water Quality Centre, South Australian Water Corporation, 250 Victoria Square, Adelaide, South Australia 5000, Australia. Phone: 61874242142. Fax: 61870032142. E-mail: daniel.hoefel{at}sawater.com.au

{triangledown} Published ahead of print on 5 June 2009.

Applied and Environmental Microbiology, August 2009, p. 5167-5169, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00036-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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