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Applied and Environmental Microbiology, August 2009, p. 5170-5174, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00254-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genome-Derived Criteria for Assigning Environmental narG and nosZ Sequences to Operational Taxonomic Units of Nitrate Reducers{triangledown} ,{dagger}

Katharina Palmer, Harold L. Drake, and Marcus A. Horn*

Department of Ecological Microbiology, University of Bayreuth, 95440 Bayreuth, Germany

Received 2 February 2009/ Accepted 1 June 2009

Ninety percent of cultured bacterial nitrate reducers with a 16S rRNA gene similarity of ≥97% had a narG or nosZ similarity of ≥67% or ≥80%, respectively, suggesting that 67% and 80% could be used as standardized, conservative threshold similarity values for narG and nosZ, respectively (i.e., any two sequences that are less similar than the threshold similarity value have a very high probability of belonging to different species), for estimating species-level operational taxonomic units. Genus-level tree topologies of narG and nosZ were generally similar to those of the corresponding 16S rRNA genes. Although some genomes contained multiple copies of narG, recent horizontal gene transfer of narG was not apparent.

* Corresponding author. Mailing address: Department of Ecological Microbiology, University of Bayreuth, 95440 Bayreuth, Germany. Phone: (49) (0)921-555620. Fax: (49) (0)921-555793. E-mail: marcus.horn{at}uni-bayreuth.de

{triangledown} Published ahead of print on 5 June 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2009, p. 5170-5174, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00254-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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