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 Previous Article

Applied and Environmental Microbiology, August 2009, p. 5175-5178, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00573-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Homo-D-Lactic Acid Fermentation from Arabinose by Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum{triangledown}

Kenji Okano,1 Shogo Yoshida,2 Tsutomu Tanaka,3 Chiaki Ogino,2 Hideki Fukuda,3 and Akihiko Kondo2*

Department of Molecular Science and Material Engineering, Graduate School of Science and Technology,1 Department of Chemical Science and Engineering, Graduate School of Engineering,2 Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan3

Received 9 March 2009/ Accepted 29 May 2009

Optically pure D-lactic acid fermentation from arabinose was achieved by using the Lactobacillus plantarum NCIMB 8826 strain whose L-lactate dehydrogenase gene was deficient and whose phosphoketolase gene was substituted with a heterologous transketolase gene. After 27 h of fermentation, 38.6 g/liter of D-lactic acid was produced from 50 g/liter of arabinose.

* Corresponding author. Mailing address: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Phone and fax: 81-78-803-6196. E-mail: akondo{at}kobe-u.ac.jp

{triangledown} Published ahead of print on 5 June 2009.

Applied and Environmental Microbiology, August 2009, p. 5175-5178, Vol. 75, No. 15
0099-2240/09/$08.00+0     doi:10.1128/AEM.00573-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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