This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Ding, C.
Right arrow Articles by Zhou, X.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, C.
Right arrow Articles by Zhou, X.
Agricola
Right arrow Articles by Ding, C.
Right arrow Articles by Zhou, X.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2009, p. 5380-5389, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.01193-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genetic Determinants of Symptoms on Viral DNA Satellites {triangledown}

Chenjun Ding, Ling Qing,{dagger} Zhenghe Li, Yi Liu, Yajuan Qian, and Xueping Zhou*

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China

Received 24 May 2009/ Accepted 16 June 2009

Begomovirus-DNA-β disease complexes induce different symptom phenotypes in their hosts. To investigate the genetic determinants of the phenotypic differences, Nicotiana spp. and tomato plants were inoculated with infectious clones of Tobacco curly shoot virus (TbCSV)/TbCSV DNA-β (TbCSB) and Tomato yellow leaf curl China virus (TYLCCNV)/TYLCCNV DNA-β (TYLCCNB) pseudorecombinants and showed that TYLCCNB induced characteristic vein-thickening and enation symptoms, while TbCSB only slightly exacerbated the leaf-curling symptoms, regardless of the helper virus being used. The roles of DNA-β-encoded βC1 and a 430-nucleotide fragment containing the A-rich region and the putative βC1 promoter region of the βC1 gene (referred to as AP) in symptom development were further investigated by constructing hybrid satellites in which the βC1 coding region or AP was exchanged between the two satellite molecules. A TYLCCNB hybrid with TbCSB βC1 lost the ability to elicit the vein-thickening and enation phenotypes. TbCSB hybrids containing the TYLCCNB βC1 or AP fragment failed to induce the characteristic vein thickening and enations. A TYLCCNB hybrid having the TbCSB AP fragment produced the enations, but the number of enations was less and their sizes were reduced. Differently from the phloem-specific pattern of the TYLCCNB promoter, a full-length fragment upstream of the TbCSB βC1 gene confers a constitutive β-glucuronidase expression pattern in transgenic tobacco plants. The above results indicate that the DNA-β-encoded βC1 protein is the symptom determinant, but the promoter of the βC1 gene has influence on symptom production.

* Corresponding author. Mailing address: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China. Phone: 86 571 86971680. Fax: 86 571 86971498. E-mail: zzhou{at}zju.edu.cn

{triangledown} Published ahead of print on 19 June 2009.

{dagger} Present address: College of Plant Protection, Southwest University, Chongqing 400716, People's Republic of China.

Applied and Environmental Microbiology, August 2009, p. 5380-5389, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.01193-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»