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Applied and Environmental Microbiology, August 2009, p. 5417-5420, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00899-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Controlled Gene Expression in the Plant Pathogen Cryphonectria parasitica by Use of a Copper-Responsive Element{triangledown}

Karyn L. Willyerd, Amanda M. Kemp, and Angus L. Dawe*

Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003

Received 21 April 2009/ Accepted 11 June 2009

We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713.


* Corresponding author. Mailing address: Department of Biology, New Mexico State University, MSC 3AF, P.O. Box 30001, Las Cruces, NM 88003. Phone: (575) 646-4003. Fax: (575) 646-5665. E-mail: dawe{at}nmsu.edu

{triangledown} Published ahead of print on 19 June 2009.


Applied and Environmental Microbiology, August 2009, p. 5417-5420, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00899-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.