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Applied and Environmental Microbiology, August 2009, p. 5424-5427, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00740-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli{triangledown}

Niju Narayanan and C. Perry Chou*

Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1

Received 31 March 2009/ Accepted 13 June 2009

Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1. Phone: (519) 888-4567, ext. 33310. Fax: (519) 746-4979. E-mail: cpchou{at}uwaterloo.ca

{triangledown} Published ahead of print on 19 June 2009.

Applied and Environmental Microbiology, August 2009, p. 5424-5427, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00740-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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