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Applied and Environmental Microbiology, August 2009, p. 5434-5436, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00949-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Plasmid-Based System for High-Level Gene Expression and Antisense Gene Knockdown in Bartonella henselae{triangledown} ,{dagger}

Devin Gillaspie,1 Izabella Perkins,1 Kellie Larsen,1 Amy McCord,1 Scott Pangonis,1 Debra Sweger,1 Mohamed N. Seleem,2 Nammalwar Sriranganathan,3 and Burt E. Anderson1*

Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, Florida 33612,1 Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,2 Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 240613

Received 27 April 2009/ Accepted 12 June 2009

Six broad-host-range plasmid vectors were developed to study gene expression in Bartonella henselae. The vectors were used to express a β-galactosidase reporter gene in B. henselae and to generate antisense RNA for gene knockdown. When applied to ompR, a putative transcription response regulator of B. henselae, this antisense RNA gene knockdown strategy reduced bacterial invasion of human endothelial cells by over 60%.

* Corresponding author. Mailing address: Department of Molecular Medicine, College of Medicine, MDC7, University of South Florida, 12901 Bruce B. Downs Blvd., Tampa, FL 33612. Phone: (813) 974-2608. Fax: (813) 974-7357. E-mail: banderso{at}health.usf.edu

{triangledown} Published ahead of print on 19 June 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2009, p. 5434-5436, Vol. 75, No. 16
0099-2240/09/$08.00+0     doi:10.1128/AEM.00949-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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