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Applied and Environmental Microbiology, September 2009, p. 5496-5500, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.01298-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product{triangledown}

Bao-zhan Wang, Peng Guo, Bao-jian Hang, Lian Li, Jian He,* and Shun-peng Li

Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China

Received 4 June 2009/ Accepted 24 June 2009

A novel esterase gene, pytH, encoding a pyrethroid-hydrolyzing carboxylesterase was cloned from Sphingobium sp. strain JZ-1. The gene contained an open reading frame of 840 bp. Sequence identity searches revealed that the deduced enzyme shared the highest similarity with many {alpha}/β-hydrolase fold proteins (20 to 24% identities). PytH was expressed in Escherichia coli BL21 and purified using Ni-nitrilotriacetic acid affinity chromatography. It was a monomeric structure with a molecular mass of approximately 31 kDa and a pI of 4.85. PytH was able to transform p-nitrophenyl esters of short-chain fatty acids and a wide range of pyrethroid pesticides, and isomer selectivity was not observed. No cofactors were required for enzyme activity.

* Corresponding author. Mailing address: College of Life Sciences, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, China. Phone: 86 2584396685. Fax: 86 2584396314. E-mail: hejian{at}njau.edu.cn

{triangledown} Published ahead of print on 6 July 2009.

Applied and Environmental Microbiology, September 2009, p. 5496-5500, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.01298-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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