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Applied and Environmental Microbiology, September 2009, p. 5523-5528, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00020-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Impact of Therapeutic Treatment with β-Lactam on Transfer of the blaCTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats{triangledown}

Stéphanie Faure, Agnès Perrin-Guyomard,* Jean-Michel Delmas, and Michel Laurentie

Laboratory for the Research and Investigation of Veterinary Drugs and Disinfectants, Pharmacokinetic-Pharmacodynamic Unit, AFSSA Fougeres, BP 90203, La Haute Marche, 35133 Javene, France

Received 6 January 2009/ Accepted 20 June 2009

The conjugative transfer of the plasmid carrying the blaCTX-M-9 gene from Salmonella enterica serovar Virchow isolated from a chicken farm to a recipient Escherichia coli strain was evaluated in vitro and in axenic rats inoculated with both strains, with or without selective pressure due to therapeutic doses of cefixime. The transfer of the blaCTX-M-9 gene of S. enterica serovar Virchow to E. coli was confirmed in vitro, at a low frequency of 5.9 x 10–8 transconjugants/donors. This transfer rate was higher in gnotobiotic rats and reached ~10–5 transconjugants/donors without selective pressure. This frequency was not affected by the addition of therapeutic doses of cefixime. Thus, estimates of in vitro transfer underestimated potential transfer in the digestive tract, and therapeutic doses of cefixime did not increase the selection for transconjugants.

* Corresponding author. Mailing address: Laboratory for the Research and Investigation of Veterinary Drugs and Disinfectants, Pharmacokinetic-Pharmacodynamic Unit, AFSSA Fougeres, BP 90203, La Haute Marche, 35133 Javene, France. Phone: 33(2) 99-94-78-78. Fax: 33(2) 99-94-78-80. E-mail: a.perrin-guyomard{at}afssa.fr

{triangledown} Published ahead of print on 6 July 2009.

Applied and Environmental Microbiology, September 2009, p. 5523-5528, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00020-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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