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Applied and Environmental Microbiology, September 2009, p. 5700-5703, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.02816-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Inter- and Intraspecies Plasmid-Mediated Transfer of Florfenicol Resistance in Enterobacteriaceae Isolates from Swine{triangledown}

Nabin Rayamajhi,1 Seung Bin Cha,1 Mi Lan Kang,1 Su In Lee,1 Hee Soo Lee,2 and Han Sang Yoo1*

Department of Infectious Disease, College of Veterinary Medicine and KRF Priority Zoonotic Disease Research Institute and Brain Korea 21 for Veterinary Science, Seoul National University, Seoul 151-742,1 Division of Bacteriology and Parasitology, National Veterinary Research and Quarantine Service, Anyang 430-757, Kyunggi, South Korea2

Received 12 December 2008/ Accepted 27 June 2009

Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, and a mixed-broth conjugation assay suggested the intra- and interspecies plasmid-mediated transfer of florfenicol resistance among the isolates that exhibited MICs for florfenicol between 4 to 128 mg/liter.

* Corresponding author. Mailing address: Department of Infectious Disease, College of Veterinary Medicine, Seoul National University, San 56-1, Shilim 9 dong, Kwanak-Ku, Seoul 151-742, South Korea. Phone: 82-2-880-1263. Fax: 82-2-874-2738. E-mail: yoohs{at}snu.ac.kr

{triangledown} Published ahead of print on 10 July 2009.

Applied and Environmental Microbiology, September 2009, p. 5700-5703, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.02816-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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