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Applied and Environmental Microbiology, September 2009, p. 5719-5726, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00558-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle{triangledown}

Noriyo Yoshii,1 Yoshitoshi Ogura,2 Tetsuya Hayashi,2 Takashi Ajiro,3 Toshiya Sameshima,4 Muneo Nakazawa,5 Masahiro Kusumoto,1 Taketoshi Iwata,1 and Masato Akiba1*

Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan,1 Division of Microbial Diseases, Department of Infectious Diseases, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan,2 Division of Livestock Industry, Department of Agriculture, Forestry and Fisheries, Miyagi Prefectural Government, 3-8-1 Honcho, Aoba-ku, Sendai, Miyagi 980-8570, Japan,3 Center for Animal Disease Control and Prevention, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan,4 Epidemiological Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan5

Received 9 March 2009/ Accepted 23 June 2009

A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

* Corresponding author. Mailing address: Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba 305-0856, Japan. Phone: 81-29-838-7815. Fax: 81-29-838-7825. E-mail: akiba{at}affrc.go.jp

{triangledown} Published ahead of print on 6 July 2009.

Applied and Environmental Microbiology, September 2009, p. 5719-5726, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00558-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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