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Applied and Environmental Microbiology, September 2009, p. 5729-5733, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00509-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

New Design Strategy for Development of Specific Primer Sets for PCR-Based Detection of Chlorophyceae and Bacillariophyceae in Environmental Samples{triangledown} ,{dagger}

Claire Valiente Moro,* Olivier Crouzet, Séréna Rasconi, Antoine Thouvenot, Gérard Coffe, Isabelle Batisson, and Jacques Bohatier

UMR 6023 Laboratoire Microorganismes: Génome et Environnement (CNRS-Université Blaise Pascal, Clermont-Ferrand II), 63177 Aubière, France

Received 2 March 2009/ Accepted 3 July 2009

Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers for Chlorophyceae and Bacillariophyceae by using the ARB software and implementing a virtual PCR program. The results of specificity analysis showed that most of the targeted algal families were identified and nontargeted organisms, such as fungi or ciliates, were excluded. These newly developed PCR primer sets were also able to amplify microalgal rRNA genes from environmental samples with accurate specificity. These tools could be of great interest for studying freshwater microalgal ecology and for developing bioindicators of the health status of aquatic environments.

* Corresponding author. Mailing address: Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement UMR CNRS 6023, 63177 Aubière cedex, France. Phone: 33 473 407453. Fax: 33 473 407670. E-mail: valienteclaire{at}yahoo.fr

{triangledown} Published ahead of print on 10 July 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2009, p. 5729-5733, Vol. 75, No. 17
0099-2240/09/$08.00+0     doi:10.1128/AEM.00509-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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