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Applied and Environmental Microbiology, September 2009, p. 5750-5760, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00040-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Microbial Biogeography of Six Salt Lakes in Inner Mongolia, China, and a Salt Lake in Argentina {triangledown} ,{dagger}

Eulyn Pagaling,1 Huanzhi Wang,1 Madeleine Venables,1 Andrew Wallace,1 William D. Grant,1 Don A. Cowan,2 Brian E. Jones,3 Yanhe Ma,4 Antonio Ventosa,5 and Shaun Heaphy1*

Department of Infection, Immunity and Inflammation, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom,1 Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa,2 Genencor International B.V., Archimedesweg 30, 2333 CN Leiden, The Netherlands,3 State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China,4 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain5

Received 8 January 2009/ Accepted 17 July 2009

We used cultivation-independent methods to investigate the prokaryotic biogeography of the water column in six salt lakes in Inner Mongolia, China, and a salt lake in Argentina. These lakes had different salt compositions and pH values and were at variable geographic distances, on both local and intercontinental scales, which allowed us to explore the microbial community composition within the context of both contemporary environmental conditions and geographic distance. Fourteen 16S rRNA gene clone libraries were constructed, and over 200 16S rRNA gene sequences were obtained. These sequences were used to construct biotic similarity matrices, which were used in combination with environmental similarity matrices and a distance matrix in the Mantel test to discover which factors significantly influenced biotic similarity. We showed that archaeal biogeography was influenced by contemporary environmental factors alone (Na+, CO32–, and HCO3– ion concentrations; pH; and temperature). Bacterial biogeography was influenced both by contemporary environmental factors (Na+, Mg2+, and HCO3– ion concentrations and pH) and by geographic distance.

* Corresponding author. Mailing address: Department of Infection, Immunity, and Inflammation, University of Leicester, University Road, Leicester LE1 9HN, United Kingdom. Phone: 44 116 252 2973. Fax: 44 116 252 5030. E-mail: sh1{at}le.ac.uk

{triangledown} Published ahead of print on 31 July 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2009, p. 5750-5760, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00040-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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