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Applied and Environmental Microbiology, September 2009, p. 5996-5998, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00643-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp. {triangledown}

A. Alagappan,1 P. L. Bergquist,1,2 and B. C. Ferrari3*

Department of Chemistry and Biomolecular Science and Environmental Biotechnology CRC, Macquarie University, Sydney, New South Wales, Australia,1 Department of Molecular Medicine and Pathology, University of Auckland Medical School, Auckland, New Zealand,2 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia3

Received 18 March 2009/ Accepted 17 July 2009

A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed. The assay is a simple, rapid, and cost-effective tool for the detection of the major Cryptosporidium species of concern to public health.

* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Phone: 612 9385 2032. Fax: 612 9385 1483. E-mail: b.ferrari{at}unsw.edu.au

{triangledown} Published ahead of print on 24 July 2009.

Applied and Environmental Microbiology, September 2009, p. 5996-5998, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00643-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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