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Applied and Environmental Microbiology, September 2009, p. 6008-6012, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00838-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Overexpression of the groESL Operon Enhances the Heat and Salinity Stress Tolerance of the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain PCC7120{triangledown}

Akhilesh Kumar Chaurasia and Shree Kumar Apte*

Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India

Received 13 April 2009/ Accepted 15 July 2009

The bicistronic groESL operon, encoding the Hsp60 and Hsp10 chaperonins, was cloned into an integrative expression vector, pFPN, and incorporated at an innocuous site in the Anabaena sp. strain PCC7120 genome. In the recombinant Anabaena strain, the additional groESL operon was expressed from a strong cyanobacterial PpsbA1 promoter without hampering the stress-responsive expression of the native groESL operon. The net expression of the two groESL operons promoted better growth, supported the vital activities of nitrogen fixation and photosynthesis at ambient conditions, and enhanced the tolerance of the recombinant Anabaena strain to heat and salinity stresses.

* Corresponding author. Mailing address: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Phone: (91) 2225595342. Fax: (91) 2225505189. E-mail: sksmbd{at}barc.gov.in

{triangledown} Published ahead of print on 24 July 2009.

Applied and Environmental Microbiology, September 2009, p. 6008-6012, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00838-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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