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Applied and Environmental Microbiology, September 2009, p. 6017-6021, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00211-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Strategy for Extracting DNA from Clay Soil and Detecting a Specific Target Sequence via Selective Enrichment and Real-Time (Quantitative) PCR Amplification {triangledown}

Kweku K. Yankson{dagger} and Todd R. Steck*

Department of Biology, The University of North Carolina at Charlotte, Charlotte, North Carolina 28223

Received 29 January 2009/ Accepted 20 July 2009

We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, µg quantities of DNA were extracted from mg samples of pure kaolinite and a field clay soil.

* Corresponding author. Mailing address: Department of Biology, The University of North Carolina at Charlotte, Charlotte, NC 28223. Phone: (704) 687-8534. Fax: (704) 687-3128. E-mail: trsteck{at}uncc.edu

{triangledown} Published ahead of print on 24 July 2009.

{dagger} Present address: Phase Forward, Clarix Products Group, Two Radnor Corporate Center, Radnor, PA 19807.

Applied and Environmental Microbiology, September 2009, p. 6017-6021, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.00211-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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