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Applied and Environmental Microbiology, September 2009, p. 6022-6025, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.01078-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Impact of Alginate Overproduction on Attachment and Biofilm Architecture of a Supermucoid Pseudomonas aeruginosa Strain{triangledown} ,{dagger}

Iain D. Hay, Kali Gatland, Andrea Campisano, J. Zoe Jordens, and Bernd H. A. Rehm*

Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand

Received 11 May 2009/ Accepted 25 June 2009

The supermucoid Pseudomonas aeruginosa strain PDO300{Delta}alg8(pBBR1MCS-5:alg8) showed strongly impaired attachment compared with the respective mucoid or nonmucoid strains and formed a thicker biofilm with large extended mushroom-like microcolonies. Alginate lyase treatment dissolved microcolonies. The data suggested that alginate overproduction impairs attachment but plays a structural role in microcolony formation.

* Corresponding author. Mailing address: Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand. Phone: 64 6 350 5515 7890. Fax: 64 6 350 5688. E-mail: B.Rehm{at}massey.ac.nz

{triangledown} Published ahead of print on 31 July 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, September 2009, p. 6022-6025, Vol. 75, No. 18
0099-2240/09/$08.00+0     doi:10.1128/AEM.01078-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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