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Applied and Environmental Microbiology, October 2009, p. 6406-6409, Vol. 75, No. 19
0099-2240/09/$08.00+0     doi:10.1128/AEM.00224-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Brevibacteriaceae by Multilocus Sequence Typing and Comparative Genomic Hybridization Analyses{triangledown} ,{dagger}

Marie-Pierre Forquin,1,2 Hugo Duvergey,3 Caroline Proux,4 Valentin Loux,3 Jerome Mounier,1,{ddagger} Sophie Landaud,1 Jean-Yves Coppée,4 Jean-François Gibrat,3 Pascal Bonnarme,1 Isabelle Martin-Verstraete,2 and Tatiana Vallaeys1,5*

INRA-AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires, Centre de Biotechnologies Agro-Industrielles, 78850 Thiverval-Grignon, France,1 Institut Pasteur, Unité de Génétique des Génomes Bactériens, URA-CNRS2171, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France,2 INRA, Unité Mathématique, Informatique et Génome UR1077, F-78352 Jouy-en-Josas, France,3 Institut Pasteur, Plate-forme Puces à ADN, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France,4 ECOLAG, UMR 5119 CNRS, Université Montpellier II, Case 093, 34095 Montpellier Cedex 5, France5

Received 30 January 2009/ Accepted 27 July 2009

Multilocus sequence typing with nine selected genes is shown to be a promising new tool for accurate identifications of Brevibacteriaceae at the species level. A developed microarray also allows intraspecific diversity investigations of Brevibacterium aurantiacum showing that 13% to 15% of the genes of strain ATCC 9174 were absent or divergent in strain BL2 or ATCC 9175.

* Corresponding author. Mailing address: Laboratoire ECOLAG, UMR CNRS-UMII 5119, Université Montpellier II, cc 093, Place E. Bataillon, 34095 Montpellier Cedex 5, France. Phone: 33 (0)4 67 14 40 11. Fax: 33 (0)4 67 14 46 74. E-mail: tatiana.vallaeys{at}univ-montp2.fr

{triangledown} Published ahead of print on 31 July 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Université Européenne de Bretagne, France, Université de Brest, EA3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, IFR148 ScInBioS, ESMISAB, Technopôle de Brest Iroise, 29280 Plouzané, France.

Applied and Environmental Microbiology, October 2009, p. 6406-6409, Vol. 75, No. 19
0099-2240/09/$08.00+0     doi:10.1128/AEM.00224-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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