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Applied and Environmental Microbiology, October 2009, p. 6410-6413, Vol. 75, No. 19
0099-2240/09/$08.00+0     doi:10.1128/AEM.00962-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genetic Basis for Alkaline Activation of Germination in Bacillus thuringiensis subsp. israelensis{triangledown}

M. M. Abdoarrahem, K. Gammon, B. N. Dancer, and C. Berry*

Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3TL, United Kingdom

Received 28 April 2009/ Accepted 28 July 2009

Differences in activation between spores from strains of Bacillus thuringiensis subsp. israelensis with and without the toxin-encoding plasmid pBtoxis are demonstrated. Following alkaline activation, the strain bearing pBtoxis shows a significantly greater germination rate. Expression of just three genes constituting a previously identified, putative ger operon from this plasmid is sufficient to produce the same phenotype and characterizes this operon as a genetic determinant of alkaline activation.

* Corresponding author. Mailing address: Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3TL, United Kingdom. Phone: 029-20874508. Fax: 029-20874305. E-mail: Berry{at}cf.ac.uk

{triangledown} Published ahead of print on 31 July 2009.

Applied and Environmental Microbiology, October 2009, p. 6410-6413, Vol. 75, No. 19
0099-2240/09/$08.00+0     doi:10.1128/AEM.00962-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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