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Applied and Environmental Microbiology, January 2009, p. 462-467, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01514-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Efficient Production of Optically Pure D-Lactic Acid from Raw Corn Starch by Using a Genetically Modified L-Lactate Dehydrogenase Gene-Deficient and {alpha}-Amylase-Secreting Lactobacillus plantarum Strain{triangledown}

Kenji Okano,1 Qiao Zhang,2 Satoru Shinkawa,2 Shogo Yoshida,3 Tsutomu Tanaka,4 Hideki Fukuda,4 and Akihiko Kondo2*

Department of Molecular Science and Material Engineering, Graduate School of Science and Technology,1 Department of Chemical Science and Engineering, Graduate School of Engineering,2 Department of Chemical Science and Engineering, Faculty of Engineering,3 Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan4

Received 4 July 2008/ Accepted 10 November 2008

In order to achieve direct and efficient fermentation of optically pure D-lactic acid from raw corn starch, we constructed L-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum and introduced a plasmid encoding Streptococcus bovis 148 {alpha}-amylase (AmyA). The resulting strain produced only D-lactic acid from glucose and successfully expressed amyA. With the aid of secreting AmyA, direct D-lactic acid fermentation from raw corn starch was accomplished. After 48 h of fermentation, 73.2 g/liter of lactic acid was produced with a high yield (0.85 g per g of consumed sugar) and an optical purity of 99.6%. Moreover, a strain replacing the ldhL1 gene with an amyA-secreting expression cassette was constructed. Using this strain, direct D-lactic acid fermentation from raw corn starch was accomplished in the absence of selective pressure by antibiotics. This is the first report of direct D-lactic acid fermentation from raw starch.

* Corresponding author. Mailing address: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Phone and fax: 81-78-803-6196. E-mail: akondo{at}kobe-u.ac.jp

{triangledown} Published ahead of print on 14 November 2008.

Applied and Environmental Microbiology, January 2009, p. 462-467, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01514-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Okano, K., Yoshida, S., Tanaka, T., Ogino, C., Fukuda, H., Kondo, A. (2009). Homo-D-Lactic Acid Fermentation from Arabinose by Redirection of the Phosphoketolase Pathway to the Pentose Phosphate Pathway in L-Lactate Dehydrogenase Gene-Deficient Lactobacillus plantarum. Appl. Environ. Microbiol. 75: 5175-5178 [Abstract] [Full Text]  


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