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Applied and Environmental Microbiology, January 2009, p. 551-556, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01527-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Isolation of Rhodococcus sp. Strain ECU0066, a New Sulfide Monooxygenase-Producing Strain for Asymmetric Sulfoxidation{triangledown} ,{dagger}

Ai-Tao Li,1 Jian-Dong Zhang,1 Jian-He Xu,1* Wen-Ya Lu,2 and Guo-Qiang Lin2

Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237,1 Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China2

Received 6 July 2008/ Accepted 23 September 2008

A new and efficient sulfide monooxygenase-producing strain, ECU0066, was isolated and identified as a Rhodococcus sp. that could transform phenylmethyl sulfide (PMS) to (S)-sulfoxide with 99% enantiomeric excess via two steps of enantioselective oxidations. Its enzyme activity could be effectively induced by adding PMS or phenylmethyl sulfoxide (PMSO) directly to a rich medium at the early log phase (6 h) of fermentation, resulting in over 10-times-higher production of the enzyme. This bacterial strain also displayed fairly good activity and enantioselectivity toward seven other sulfides, indicating a good potential for practical application in asymmetric synthesis of chiral sulfoxides.

* Corresponding author. Mailing address: Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Phone: 86-21-6425-2498. Fax: 86-21-6425-2250. E-mail: jianhexu{at}ecust.edu.cn

{triangledown} Published ahead of print on 3 October 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2009, p. 551-556, Vol. 75, No. 2
0099-2240/09/$08.00+0     doi:10.1128/AEM.01527-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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