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Applied and Environmental Microbiology, October 2009, p. 6613-6615, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.01587-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Inducible-Expression Plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans{triangledown} ,{dagger}

Alice C. Ind,1,2,{ddagger} Steven L. Porter,1,2,{ddagger} Mostyn T. Brown,2 Elaine D. Byles,1,2 Jennifer A. de Beyer,1 Scott A. Godfrey,3,4 and Judith P. Armitage1,2*

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom,1 Oxford Centre for Integrative Systems Biology, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom,2 School of Biological and Molecular Sciences, Oxford Brookes University, Headington OX3 0BP, United Kingdom,3 Centre for Research in Plant Science, Faculty of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, United Kingdom4

Received 6 July 2009/ Accepted 10 August 2009

We have developed a stable isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacIq gene from pYanni3 and the lac promoter, PA1/04/03, from pJBA24.

* Corresponding author. Mailing address: Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom. Phone: 44 (0)1865 613293. Fax: 44 (0)1865 613338. E-mail: judith.armitage{at}bioch.ox.ac.uk

{triangledown} Published ahead of print on 14 August 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} A.C.I. and S.L.P. contributed equally to this study.

Applied and Environmental Microbiology, October 2009, p. 6613-6615, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.01587-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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