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Applied and Environmental Microbiology, October 2009, p. 6619-6621, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.01136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Particle Size Classes Inhibiting Protozoan Recovery from Surface Water Samples via U.S. Environmental Protection Agency Method 1623{triangledown}

Leigh-Anne H. Krometis,* Gregory W. Characklis, and Mark D. Sobsey

Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, Rosenau Hall, CB7431, University of North Carolina, Chapel Hill, North Carolina 27599-7431

Received 17 May 2009/ Accepted 5 August 2009

Giardia species recovery by U.S. Environmental Protection Agency method 1623 appears significantly impacted by a wide size range (2 to 30 µm) of particles in water and organic matter. Cryptosporidium species recovery seems negatively correlated only with smaller (2 to 10 µm), presumably inorganic particles. Results suggest constituents and mechanisms interfering with method performance may differ by protozoan type.

* Corresponding author. Present address: Department of Biological Systems Engineering, 200 Seitz Hall (0303), Virginia Tech, Blacksburg, VA 24061-0303. Phone: (540) 231-4372. Fax: (540) 231-3199. E-mail: krometis{at}vt.edu

{triangledown} Published ahead of print on 14 August 2009.

Applied and Environmental Microbiology, October 2009, p. 6619-6621, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.01136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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