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Applied and Environmental Microbiology, October 2009, p. 6622-6625, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.00639-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cloning of Salmonella enterica Serovar Enteritidis Fimbrial Protein SefA as a Surface Protein in Escherichia coli Confers the Ability To Attach to Eukaryotic Cell Lines{triangledown}

Douglas L. Rank,1 Mahdi A. Saeed,2 and Peter M. Muriana3*

Department of Animal Science, Oklahoma State University, Stillwater, Oklahoma 74078,3 Department of Epidemiology, Michigan State University, East Lansing, Michigan 48824,2 Department of Food Science, Purdue University, West Lafayette, Indiana 479071

Received 18 March 2009/ Accepted 14 August 2009

The gene for the Salmonella enterica serovar Enteritidis fimbrial protein SefA was cloned into an Escherichia coli surface expression vector and confirmed by Western blot assay. E. coli clones expressing SefA attached to avian ovary granulosa cells and HEp-2 cells, providing evidence for the involvement of SefA in the ability of Salmonella to attach to eukaryotic cells.

* Corresponding author. Mailing address: Robert M. Kerr Food & Agricultural Products Center, 109 FAPC Bldg., Oklahoma State University, Stillwater, OK 74078-6055. Phone: (405) 744-5563. Fax: (405) 744-6313. E-mail: peter.muriana{at}okstate.edu

{triangledown} Published ahead of print on 21 August 2009.

Applied and Environmental Microbiology, October 2009, p. 6622-6625, Vol. 75, No. 20
0099-2240/09/$08.00+0     doi:10.1128/AEM.00639-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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