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Applied and Environmental Microbiology, November 2009, p. 6745-6756, Vol. 75, No. 21
0099-2240/09/$08.00+0 doi:10.1128/AEM.01171-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Rapid Identification of Vibrio parahaemolyticus by Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
,
Tracy H. Hazen,1,
Robert J. Martinez,1,
Yanfeng Chen,2
Patricia C. Lafon,3
Nancy M. Garrett,3
Michele B. Parsons,3
Cheryl A. Bopp,3
M. Cameron Sullards,1,2 and
Patricia A. Sobecky1*
School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332,1 School of Chemistry and Biochemistry, The Parker H. Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332,2 Enteric Diseases Laboratory Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303333
Received 20 May 2009/ Accepted 3 September 2009
Vibrio parahaemolyticus is a pathogenic marine bacterium that is the main causative agent of bacterial seafood-borne gastroenteritis in the United States. An increase in the frequency of V. parahaemolyticus-related infections during the last decade has been attributed to the emergence of an O3:K6 pandemic clone in 1995. The diversity of the O3:K6 pandemic clone and its serovariants has been examined using multiple molecular techniques including multilocus sequence analysis, pulsed-field gel electrophoresis, and group-specific PCR analysis. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has become a powerful tool for rapidly distinguishing between related bacterial species. In the current study, we demonstrate the development of a whole-cell MALDI-TOF MS method for the distinction of V. parahaemolyticus from other Vibrio spp. We identified 30 peaks that were present only in the spectra of the V. parahaemolyticus strains examined in this study that may be developed as MALDI-TOF MS biomarkers for identification of V. parahaemolyticus. We detected variation in the MALDI-TOF spectra of V. parahaemolyticus strains isolated from different geographical locations and at different times. The MALDI-TOF MS spectra of the V. parahaemolyticus strains examined were distinct from those of the other Vibrio species examined including the closely related V. alginolyticus, V. harveyi, and V. campbellii. The results of this study demonstrate the first use of whole-cell MALDI-TOF MS analysis for the rapid identification of V. parahaemolyticus.
* Corresponding author. Present address: 300 Hackberry Lane, Tuscaloosa, AL 35401. Phone: (205) 348-8330. Fax: (205) 348-1786. E-mail: psobecky{at}bama.ua.edu
Published ahead of print on 11 September 2009.
Supplemental material for this article may be found at http://aem.asm.org/.
Present address: Enteric Diseases Laboratory Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Present address: Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35401.
Applied and Environmental Microbiology, November 2009, p. 6745-6756, Vol. 75, No. 21
0099-2240/09/$08.00+0 doi:10.1128/AEM.01171-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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